Microbiological laboratory

The microbiological laboratory is equipped with modern equipment, Culture Media, test systems, reagents and two microbiological computer analyzers: Micro-Tax (Austria) and Mini API (France). Allows you to identify a wide range of pathogens of infectious and purulent-inflammatory diseases, as well as quickly identify clinically important facultative-anaerobic bacteria in case of urgent need (within 3-5 hours).

The microbiological laboratory is part of the diagnostic services of JSC" NCSC".

  • Established in 2001.
  • The action is located in a separate building on the territory of the hospital complex.
  • Permission of the regime Commission to work with infectious material of pathogenicity groups III-IV No. kz08vmy00003039 dated 26.04.2022.
  • The laboratory's activities are carried out in clinical, scientific and educational areas.
  • The laboratory includes a Department of bacteriological and serological diagnostics.

The microbiological laboratory is equipped with modern equipment, Culture Media, test systems, reagents and three microbiological computer analyzers: Vitek-2 Compact (Biomerieux Russia) and Bact/Allert (Biomerieux Russia), which makes it possible to identify a wide range of pathogens of infectious and purulent-inflammatory diseases, as well as, if urgently needed, conduct rapid identification (within 3-5 hours) and sensitivity of clinically important facultative-anaerobic bacteria to antibiotics.

  • Good material and technical base: (microbiological computer analyzers" Vitek Compact", automatic bacteriological analyzer of blood cultures bact/ALERT® 3D, reagents, Metrological control).
  •  Personnel potential (all doctors and laboratory assistants have the highest category and have been working for more than 15 years).
  • The use of standardized antibiotic sensitivity determination and testing methods (EUCAST).
  • Conduct regular intra-laboratory monitoring.
  • VOC-participation in External international quality control and inter-laboratory comparison studies.
  • The WHONET program, mis and Fox systems were also introduced.


*Vitek 2 Compact Analyzer is a fully automatic system that provides the detection of microorganisms and their antimicrobial sensitivity in one day.

*The system is designed to detect gram-negative Bacillus, gram-positive cocci, anaerobic bacteria, neisseries, hemophilic Bacillus, other stubborn bacteria, Corynebacteria, lactobacilli, Bacillus, fungi (more than 450 taxa).

* Advanced Expert System the built-in expert system automatically checks each study result for sensitivity, providing a specific phenotype profile, indicating the resistance mechanism/s for each isolate.

* BACT / ALERT® 3D - automatic bacteriological analyzer of blood cultures.

INTRA-LABORATORY QUALITY CONTROL

Escherichia coli ATCC 25922

Pseudomonas aeruginosa ATCC 27853

Staphylococcus aureus ATCC 29213

Enterococcus faecalis ATCC 29212

EXTERNAL QUALITY ASSESSMENT

2015 – 2022 - The program of inter-laboratory comparison tests in the Department" Clinical Microbiology "of the ASNP" Center for external quality control of clinical laboratory tests".)


INTRODUCTION OF INNOVATIVE DIAGNOSTIC METHODS


The microbiological laboratory has implemented the whonet computer analytics program (who) to monitor microbial landscape and amp Resistance

Caesar is a participant in the antimicrobial resistance surveillance network in Central Asia and Eastern Europe project and the European antimicrobial resistance surveillance network (EARS-Net) organized by ECDC.


Determination and interpretation of antibiotic sensitivity results is carried out in accordance with the recommendations of EUCAST-European amp sensitivity Determination Committee

Participant of a multicentric study " assessment of the sensitivity of clinical isolates to cetazidime/avibactam in Russia. CAZAVI-3" (Smolensk, 2021)

 

THE LEVEL OF PRODUCTIVITY OF SCIENTIFIC ACTIVITY

Name

Years

2019

2022

1

Articles / peer-reviewed journals

6/ (2)

5/0

2

Theses / international.

6 / 0

2 /2

3

Reports at conferences/international

4/0

12/3

4

Conducting master classes

2

3

5

Scientific adviser to the doctoral student of JSC "MUA"

1

1

6

Scientific adviser to the master's student of JSC "MUA"

1

1

 

STRENGTHS

  • All medical and secondary medical personnel of the laboratory have the highest qualification category in the specialty.
  • This is the personnel stability of the laboratory(doctors and laboratory assistants have been working in the laboratory for more than 15 years, and 3 employees have been working since the date of its establishment).
  • High quality analysis.
  • Stable moral and psychological climate
  • Participation in international programs for External Quality Assessment in Clinical Microbiology (UK, Russia)
  • in the microbiological laboratory of JSC "NSMC", a computer analytical program whonet was introduced to monitor the microbial landscape and resistance of microorganisms to amp.
  • Also participation in the international Caesar Program (program for monitoring resistance to antimicrobial drugs in Central Asia and Eastern Europe) - the microbiological laboratory of JSC "NCSMO" in the Republic of Kazakhstan was the first to join this program.

    MICROBIOLOGICAL MONITORING IN A MULTIDISCIPLINARY CLINIC ACCORDING TO INTERNATIONAL STANDARDS PROVIDES:
  • To identify the dominant pathogens of purulent-inflammatory diseases in the clinic (according to WHO,2017).
  • Implementation of rational antibiotic therapy based on the identified antibioticograms.
  • Timely implementation of anti-epidemic measures.

MICROBIOLOGICAL LABORATORY:

  • diagnostic microbiological analyzes according to the gobMp
  • laboratory support of nosocomial infection control
  • diagnostic microbiological analyzes under the conditions
  • examination of clinic staff (medical examinations)

LIST OF SERVICES PROVIDED BY THE MICROBIOLOGICAL LABORATORY: 

 BACTERIOLOGICAL RESEARCH:

  1. manual bacteriological examination of autopsy material
  2. bacteriological study of biological material Vibrio cholerae (vibriocholera) (manual (isolation of pure culture)
  3. manual study of biological material for fungi of the genus Candida (candida) (isolation of pure culture)
  4. manual bacteriological study of biological material for fungi of the genus Aspergillus (isolation of pure culture)
  5. bacteriological study of biological material for fungi of the genus candida (candida) in the Analyzer
  6. manual bacteriological examination of biological material for food toxicoinfection (isolation of pure culture)
  7. manual bacteriological examination of breast milk (pure culture isolation)
  8. manual bacteriological study of breast milk on an analyzer (isolation of pure culture)
  9. manual bacteriological examination of feces for listeriosis (isolation of pure culture)bacteriological research of dysbacteriosis
  10. manual bacteriological examination of feces for microbiocenosis of the vagina
  11. manual bacteriological examination of feces for microbiocenosis of the oral cavity
  12. manual bacteriological examination of feces for pathogenic and conditionally pathogenic microflora (isolation of pure culture)
  13. bacteriological study of blood for manual salmonellosis (isolation of pure culture)
  14. bacteriological study of blood for manual sterility (isolation of pure culture)
  15. bacteriological examination of blood for sterility in an analyzer with a basic method of bacteriological research (selection of pure culture)
  16. bacteriological examination of sputum in the Analyzer (isolation of pure culture)
  17. bacteriological study of nasopharyngeal mucus Neisseria meningitis (isolation of pure culture) manually
  18. bacteriological examination of cerebrospinal fluid in Neisseria meningitis (pure culture isolation) manually
  19. bacteriological study of nasopharyngeal mucus Neisseria meningitis (Neisseria meningitis) on the Analyzer
  20. bacteriological study of secretions from the pharynx and nose in Staphylococcus aureus (isolation of pure culture)
  21. bacteriological study of Staphylococcus aureus (Staphylococcus aureus) secreted from the throat and nose in the Analyzer
  22. bacteriological study of pharyngeal secretions on Bordetella pertussis (isolation of pure culture)
  23. bacteriological examination and sensitivity to antibiotics Group B Streptococcus (Str. agalastiae)
  24. bacteriological study of biomaterial in MRSA (methicillin-resistant Staphylococcus aureus)
  25. bacteriological study of biomaterial in VRE (vancomycin-resistant Enterococcus)
  26. bacteriological examination of the pharynx, wounds, eyes, ears, urine, bile, etc. by manual method (isolation of pure culture)
  27. bacteriological examination of secretions from the pharynx, wounds, eyes, ears, urine, bile, etc. on the Analyzer
  28. bacteriological examination of the nose and pharynx with manual diphtheria (isolation of pure culture)
  29.  bacteriological examination of the nose and pharynx for diphtheria on the analyzer with the help of a simple method of bacteriological research of smyvov from the bronchi (selection of pure culture)
  30. bacteriological study of cerebrospinal fluid on Neisseria meningitidis (Neisseria meningitis) (isolation of pure culture) by manual method
  31. manual (without separation of pure culture)bacteriological examination of feces for pathogenic and conditionally pathogenic microflora
  32.  manual creation of the Wasserman reaction in blood serum

    SEROLOGICAL RESEARCH:
  33. manual staging of microprecipitation reaction with serum cardiolipin antigen
  34. staging the Wright reaction in blood serum for brucellosis
  35. setting the serum heddelson reaction to brucellosis
  36. staging a passive hemagglutination (RPGA) reaction to serum yersiniosis
  37. staging a passive hemagglutination (RPGA) response to serum whooping cough
  38. staging a passive hemagglutination (RPGA) response to serum pseudotuberculosis
  39. staging a passive hemagglutination (RPGA) reaction to serum paracoclush
  40. staging a passive hemagglutination (RPGA) reaction to serum salmonellosis
  41. staging a passive hemagglutination (RPGA) response to serum listeriosis
  42. staging a passive hemagglutination (RPGA) reaction to serum pasteurellosis

    SANITARY AND BACTERIOLOGICAL RESEARCH:
  43. bacteriological study of leeches in BSCP, pathogenic flora, Staphylococcus (one study)
  44. bacteriological study of the air of the premises 1) total microbial number (CNS) 2) pathogenic Staphylococcus 3) fungi
  45. bacteriological study of medicines for sterility (one study)
  46. bacteriological examination of the suture, bandage material material tool, hands for sterility (one study)
  47. bacteriological assessment of the safety and effectiveness of disinfectants (one study)
  48. bacteriological control of disinfection and sterilization equipment (sterilizers, decamers) one study
  49. obtaining material for microbiological research
  50. quality control of the culture medium

Address:

42 Abylai Khan Ave., Astana, Republic of Kazakhstan (one-story detached building)

Mail: 

microb_nnmc@mail.ru

Working hours:

Working days: 08:00 - 17: 00

Saturday: 08: 00-14: 00

Day off: Sunday

Taking the analysis:

Working days: from 8: 00 to 16: 00

Saturday: 08: 00-13: 00

Communication

Place of registration: +7 (7172) 57-74-40

Watsap: +7 (702) 094-77-73

Head: +7 (7172) 57-43-72


Талдау нәтижелерін алу/Получить результаты анализов

Мекен-жайы/Адрес

Казахстан, г.Астана, пр. Абылайхана 42

Телефон

Почта

national_clinic@nnmc.kz

Прейскурант

Науқастарға/Пациентам